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Titlebook: Shotgun Proteomics; Methods and Protocol Mónica Carrera,Jesús Mateos Book 2021 Springer Science+Business Media, LLC, part of Springer Natur

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SWATH-MS Protocols in Human Diseasesheir origin and early progression. Thus, it is a key factor in better diagnosis, prognosis, and treatment. Proteomics can help to solve the problem of sample complexity when the most common primary sample specimens were analyzed: organic fluids of easy access. The latest developments in high-through
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Serum Proteomic Profiling in Rheumatoid Arthritis by Antibody Suspension Bead Arraysoarrays are becoming very useful tools to identify candidate biomarkers in human body fluids for disease states such as rheumatoid arthritis (RA). In RA serum, there is a high prevalence of rheumatoid factor (RF), which is an antibody with high specificity against Fc portion of IgG. The presence of
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Proteogenomic Approach for , Investigationomics, and it allows to correctly translate start and stop sites and to reveal new open reading frames. Here, we focus on using proteogenomics to improve the annotation of . strains. We also describe detail procedures of the extraction of proteins and their further preparation for LC–MS/MS analysis
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Shotgun Proteomics for Food Microorganism Detectionensive. Liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS/MS) has been used for the analysis of bacterial pathogen strain-specific diagnostic peptides allowing the characterization of bacterial strains..Here, we describe the analysis of tryptic digestion peptides by L
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