Titlebook: Service-orientierte Architekturen; Chancen und Herausfo Volker Nissen,Mathias Petsch,Hagen Schorcht Book 2008 Gabler Verlag | Springer Fach

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f kinase inhibitors both for research and clinical use. Written in the highly successful .Methods in Molecular Biology™. series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, a

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Sven Overhage,Klaus Turowskif kinase inhibitors both for research and clinical use. Written in the highly successful .Methods in Molecular Biology™. series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, a

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Falk Kretzschmar,Ralf PetersPI4Ks). Using PI4Ks as an example, we also provide a detailed protocol for small-scale kinase expression and affinity purification from transiently transfected mammalian cells. For measuring lipid kinase activity we apply a universal bioluminescent ADP detection approach. The approach is compatible

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wing cell fixation while also preserving the fluorescence of a stably expressed fluorescent protein-tagged reporter for CDK2 activity. We provide methodology for data extraction and handling including an R-script that converts the multidimensional data into four simple binary outcomes, on which a hi

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Jan vom Brocke,Christian Sonnenberguggability of the nucleotide-binding site. This assay takes advantage of a change in fluorescence arising when the dye, SYPRO Orange, binds to hydrophobic patches that become exposed when a protein undergoes thermal unfolding. Ligand binding to a protein is known to increase its thermal stability, w

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Jochen Müller,Florian Jostock,Joachim Götze,Paul Mülleruggability of the nucleotide-binding site. This assay takes advantage of a change in fluorescence arising when the dye, SYPRO Orange, binds to hydrophobic patches that become exposed when a protein undergoes thermal unfolding. Ligand binding to a protein is known to increase its thermal stability, w

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ls kind. Vanaf de jaren tachtig werd meer gevolg gegeven aan meldingen en steeg het aantal aangiften bij politie en justitie. Deze studie geeft een eerste inzicht in het fenomeen ‘seksueel misbruik van pleegkinderen in pleegzorg’. Meer studies zijn nodig, in het bijzonder over karakteristieken van d

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