Titlebook: Sepsis; Diagnostic Methods a Nicasio Mancini Book 2015 Springer Science+Business Media New York 2015 biomarkers.blood culture.broad-range P

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Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based I years, techniques for the identification of bacteria directly from positive blood cultures have been developed (Lagace-Wiens et al., J Clin Microbiol 50:3324–3328, 2012; Martiny et al., Eur J Clin Microbiol Infect Dis 31:2269–2281, 2012; Moussaoui et al., Clin Microbiol Infect 16:1631–1638, 2010).

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Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated successful and sensitive downstream analysis is the efficient DNA extraction step. In recent years, a number of in-house and commercial DNA extraction solutions have become available. Careful evaluation in respect to cell wall disruption of various microbes and subsequent recovery of microbial DNA w

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Broad-Range PCR in the Identification of Bacterial and Fungal Pathogens from Positive Blood Culture prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification procedures..Sequence analysis of the 16S rRNA gene is a widely accepted tool for molecular identification of bacteria. Pyrosequencing is a DN

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Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-d are associated with improved patient outcomes, improved antimicrobial stewardship, improved infection control, and reduced healthcare costs. Microarrays serve as reliable platforms for the identification of these bloodstream pathogens and their associated antimicrobial resistance genes, if present

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Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols,method has to be able to fulfill several fundamental requirements for optimal performance of diagnostic assays. First of all, low- and high-molecular-weight substances of the blood inhibitory to downstream analytical reactions like PCR amplification have to be removed. This includes human DNA which

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Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols,tomated systems for the molecular diagnosis of microorganisms directly in blood samples is at its beginning. Important characteristics of systems demanded for routine use include high recovery of microbial DNA, DNA-free containment for the reduction of DNA contamination from exogenous sources, DNA-f

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Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples,given the fact that a timely diagnosis is the decisive factor to the survival of patients with bloodstream infections..Multiplex real-time PCR is a quantitative method for simultaneous amplification and detection of different targeted DNA molecules within hours. Nevertheless, various studies have sh