Titlebook: Selected Topics in Mass Spectrometry in the Biomolecular Sciences; Richard M. Caprioli,Antonio Malorni,Giovanni Sindo Book 1997 Springer S

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Protein Characterization by MS in the Pharmaceutical Industry of reversed phase HPLC with electrospray ionization mass spectrometry (LC-ESMS) was found sufficiently sensitive to allow . of such congeners at low concentrations in batch samples despite the presence of the large bulk of r-HV1 and the relatively high concentration of ammonium acetate necessary as

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Combined Analyzer Technologiesbility to analyze larger molecules induced again the development of new ionization methods, thus adding “soft” ionization techniques to the commonly used electron impact ionization (EI). Methods like chemical ionization (CI), field desorption (FD), and fast atom bombardment (FAB) opened a wide research field for mass spectrometry [7–10].

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Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry: Principles and Applicationsible mass range is > 500 000 Da, a mass accuracy of < 50 ppm for peptides < 2000 Da can be reached, the sample amounts required are 1 pmol or less. Proteins, glycoproteins, oligonucleotides and oligosacharides can be analyzed.

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Quantitative Analysis by Mass Spectrometry: Some Important Considerationsative mass spectrometry. This chapter outlines some of the basic principles, but space limitations preclude a detailed coverage; instead, several of the issues specific to biological mass spectrometry are covered and references to relevant literature are provided.

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Interaction of Metal Ions and Protons with Simple Biomolecules. Structure Information from Bond EnerThe proton affinities of N- and C-terminal breakup products of protonated peptides are also evaluated by the kinetic method, in an effort to gain new insight on the decomposition mechanism of peptide ions.

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Time-of-Flight Mass Spectrometrye for subjects not covered here. [1–3] We begin with a brief history covering some of the seminal developments, proceed to an overview of TOF theory and practice, and then discuss the present state-of-the-art of TOF instruments for biological applications. The discussion concludes with a brief (and perhaps cloudy) gaze into the future.