Titlebook: Schizosaccharomyces pombe; Methods and Protocol Teresa L. Singleton Book 2018 Springer Science+Business Media, LLC, part of Springer Nature

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The No-Nonsens SDS-PAGE,ies but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.

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Antibody Pull-Down Experiments in Fission Yeast, other proteins. Knowing the binding partners of a protein is instrumental for understanding its function. The antibody pull-down method is a powerful and common approach to detect protein-protein interactions. Here, an antibody pull-down protocol is described for detecting protein-protein interacti

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Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation,hly specific interaction between antigen and antibody, a target protein is purified and concentrated effectively. To obtain reasonable amounts of a target protein, it is crucially important to prepare total cell lysates in which the target protein is present in a soluble form. Here, we describe meth

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High-Frequency Lithium Acetate Transformation of ,,stem. We describe here an efficient transformation procedure for use in the fission yeast .. This method relies on chemical agents (lithium acetate, and polyethylene glycol) and temperature stresses, which ultimately facilitate transfer of the genetic material through the cell wall and plasma membra

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Tetrad Dissection in Fission Yeast,le to determine linkage, identify unique phenotypes associated with double mutants, or assess specific meiotic defects. Strains are crossed on nitrogen-limiting medium for 3 days. With the help of a micromanipulator, ripe asci are isolated to spots 5 mm apart on a YES plate. Incubation at 36 °C for