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Titlebook: Schistosoma mansoni; Methods and Protocol David J. Timson Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Schi

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Book 2020s to interrogate the cell biology of the worm, protocols to look at proteome level changes, prepare and work with specific proteins from .S. mansoni, .describes a drug screening, and how to test potential vaccine targets. Written in the highly successful .Methods in Molecular Biology .series format,
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Luminescence-Based, Low- and Medium-Throughput Assays for Drug Screening in , Larval Stage,medium-high scale. The assay is based on the quantitation of the parasite ATP, a good indicator of metabolically active cells, as measure of schistosomula viability. This assay is fast and reproducible, and it is suitable either for manual or for semiautomated screenings.
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Testing of , Vaccine Targets,transmission blocking (fecal egg expulsion and egg hatching rates) efficacies for the vaccine target. The RNA sequencing approaches provide basis for identification of molecular signatures predictive of desirable outcomes for schistosomiasis vaccines.
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Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult , Using DIG-Labeled RNA ence detection of gene transcripts (mRNAs) in semithin sections of . adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect
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