Titlebook: Sample Preparation in Biological Mass Spectrometry; Alexander R. Ivanov,Alexander V. Lazarev Book 2011 Springer Science+Business Media B.V

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Pressure-Assisted Lysis of Mammalian Cell Cultures Prior to Proteomic Analysisolecular mechanisms of cellular processes. Good control, efficiency, and reproducibility of protein extraction from cells and tissues are essential aspects for downstream proteomic assays, as well as for other diverse biological and basic research applications. However, common cell lysis techniques

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Development of an On-Bead Digestion Procedure for Immunoprecipitated Proteinspowerful method to determine the composition of interacting proteins and protein complexes. Receptor proteins and insoluble complexes offer added challenges, as they may contain hydrophobic membrane proteins. When strong detergents such as SDS are required to release the complexes from the magnetic

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Exploring the Capabilities of the Protein Identification by Unconventional Sample Preparation Approat the growing field of proteomics faces. A robust and efficient digest of separated proteins is central to the development of any quantitative and/or qualitative proteomic approach. We describe here two unconventional approaches to a trypsin digestion of biological samples. One of those approaches i

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Brain Proteomics: Sample Preparation Techniques for the Analysis of Rat Brain Samples Using Mass Spemark on direct analysis of proteins in the brain sections. In this protocol chapter, we provide the details on the sample preparation methods for brain proteins analysis by mass spectrometry. Sample preparation methods include first grinding the brain and/or its regions in liquid nitrogen, followed

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Subcellular Fractionation Using Step-Wise Density Extraction for Mass Spectrometry Analysishput. However, the ability to generate quantitative information on complex biological samples, especially for identification of low abundance proteins, subcellular compartment-specific protein expression and biomarker discovery, still depends upon the quality and the reproducibility of the sample pr