Titlebook: Salamanders; Methods and Protocol Ashley W. Seifert,Joshua D. Currie Book 2023 The Editor(s) (if applicable) and The Author(s), under exclu

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Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvaemogen or fluorescence-based detection. Here we describe a protocol for the spatiotemporal analysis of mRNA transcripts in axolotl embryos/larvae using digoxigenin-labeled riboprobes, anti-digoxigenin alkaline phosphatase, Fab fragments antibody, and NBT/BCIP chromogen detection.

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Methods for Studying Appendicular Skeletal Biology in Axolotlsprotocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.

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The Use of Small Molecules to Dissect Developmental and Regenerative Processes in Axolotls use of small molecules to target specific signaling pathways allows studies to proceed in animals that are difficult to manipulate genetically. This chapter provides a description of how we administer these chemicals to axolotls.

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Salamanders978-1-0716-2659-7Series ISSN 1064-3745 Series E-ISSN 1940-6029