Titlebook: Salamanders; Methods and Protocol Ashley W. Seifert,Joshua D. Currie Book 2023 The Editor(s) (if applicable) and The Author(s), under exclu

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Establishing a New Research Axolotl Colonynerates amputated limbs and injured body parts. Recent progress in research tool development has also made possible axolotl genetic manipulation and single-cell analysis, which will help understand the molecular mechanisms of complex tissue regeneration. To support the growing popularity of this mod

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EWE

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Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvaetide probe permeable tissue samples. This methodology involves the hybridization of an antisense nucleotide probe to the target mRNA, followed by chromogen or fluorescence-based detection. Here we describe a protocol for the spatiotemporal analysis of mRNA transcripts in axolotl embryos/larvae using

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Ethyl Cinnamate-Based Tissue Clearing Strategiesis due to the refractive index mismatch between its three major constituent components (lipids, proteins, and water). All tissue clearing methods rectify this mismatch by homogenizing the refractive index within the tissue and carefully matching it to the surrounding media. Here we describe a detail

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