Titlebook: SMC Complexes; Methods and Protocol Anjana Badrinarayanan Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Chro

NO610

书目名称SMC Complexes影响因子(影响力)




书目名称SMC Complexes影响因子(影响力)学科排名




书目名称SMC Complexes网络公开度




书目名称SMC Complexes网络公开度学科排名




书目名称SMC Complexes被引频次




书目名称SMC Complexes被引频次学科排名




书目名称SMC Complexes年度引用




书目名称SMC Complexes年度引用学科排名




书目名称SMC Complexes读者反馈




书目名称SMC Complexes读者反馈学科排名

低三下四之人

1064-3745 expertsThis book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic .S.tructural .M.aintenance of .C.hromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function,

Exclude

Knocking in Multifunctional Gene Tags into SMC Complex Subunits Using Gene Editingified and localized (multifunctional tags). In this chapter, we detail how to engineer vertebrate cells using CRISPR/Cas9 to provide researchers powerful tools to obtain greater precision than ever to understand how the complex interacts and behaves in cells.

新鲜

Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenanceir mechanism and impact on genome organization in a cellular context. Here, I describe a Hi-C protocol, a variant of C techniques, to construct genome-wide DNA contact maps for bacteria. This protocol is optimized for the gram-negative bacterium ., but it can be readily adapted for any bacterial species of interest.

Blood-Vessels

lethal

Pillory

正常

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein–Protein Interactions in SMC Cots modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.

蛛丝

Using Cell Cycle-Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural functions, chromatin binding pattern and functional interactions. Here, we describe the generation of the cell cycle-restricted mutants in diploid and haploid cells and the validation of their functionality with several approaches.

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