Titlebook: Rolling Circle Amplification (RCA); Toward New Clinical Vadim V. Demidov Book 2016 Springer International Publishing Switzerland 2016 Coup

Chromatic

碎石头

Nicking Enzyme-Assisted Branched-Chain RCA Reaction for Cascade DNA Amplificationnonlinear RCA format by introducing a nicking enzyme into the hyperbranched RCA system. This innovative DNA amplification technique, named as netlike RCA (NRCA) and described in this chapter, resulted in cubic amplification of a HIV-1 DNA target sequence and strongly discriminated it from its single-base mutants.

态度暖昧

CHYME

古老

,Introduction: 20+ Years of Rolling the DNA Minicircles—State of the Art in the RCA-Based Nucleic Acces the concept of RCA and discusses its plausible mechanism. It also outlines the unique advantages in molecular biotechnology and molecular medicine offered by RCA, some of which are not covered by this book.

palpitate

Glutinous

,Introduction: 20+ Years of Rolling the DNA Minicircles—State of the Art in the RCA-Based Nucleic Acdopted widely. Presently, the RCA-based methods become a broadly used DNA amplification tool and an inexpensive isothermal alternative to still dominant thermally cycled PCR, which is important when restrictions on cost and/or elaborate hardware are an issue. Not less important is that RCA reactions

利用

Improvement of ϕ29 DNA Polymerase Amplification Performance by Fusion of DNA Binding Motifsicate the viral genome from a single DNA binding event without the assistance of helicases and processivity factors. These two features, together with high nucleotide insertion fidelity, led to the development of isothermal double-stranded (ds) DNA amplification protocols. They were based on the com

Mirage

photophobia

Use of DNA CircLigase for Direct Isothermal Detection of Microbial mRNAs by RNA-Primed Rolling Circlach to detection of RNA by reverse transcription polymerase chain reaction (RT-PCR). RP-RCA significantly simplifies detection of microbial mRNA and dramatically reduces false-positive signals. Circular DNA probe is a necessary component for RP-RCA, and its preparation is crucial for the successful