Titlebook: Rice Genome Engineering and Gene Editing; Methods and Protocol Anindya Bandyopadhyay,Roger Thilmony Book 2021 Springer Science+Business Med

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TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology complex agronomic traits in plants. Here we present a high-efficiency multigene assembly and transformation vector system, TransGene Stacking II (TGS II), for these purposes. The operation process is described in detail, and the successful operation mainly depends on effective reagents, special . s

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Morphogenic Regulators and Their Application in Improving Plant Transformationnsformation methods are the most commonly used technology but these approaches are limited to a small number of plant species with efficient transformation systems. The ideal transformation technologies are those that allow biotechnology applications across wide genetic background, especially within

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-Mediated Transformation of Rice by , (,) Gene Containing CRISPR/Cas9 Vector .-mediated transformation (AMT) is considered a suitable gene transformation method. The AMT for .-type rice is a challenge because it is less efficient in tissue culture response than .-type rice. Here is a protocol of the AMT method that we developed for IR64 variety which has been successfully t

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Genotype-Independent Regeneration and Transformation Protocol for Rice Cultivarsnetic enhancement of crops, especially rice. The present study has been carried out in order to establish a genotype-independent regeneration and biolistic transformation protocol for rice varieties. Putative transgenic rice lines were confirmed by PCR analysis, DNA sequencing, and Southern analysis

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Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvementimal. This technology can be harnessed with ease to understand the basic genetics of a living system by altering sequence of individual genes and characterizing their functions. The precision of this technology is unparallel. It allows very precise and targeted base pair level edits in the genome. H

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Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice focused on gene knocking out by creating indels. However, more precise genome editing tools are demanded to assist the introduction of functional single nucleotide polymorphisms (SNPs) in breeding programs. The CRISPR base editing tools were developed to meet this need. In this chapter, we present

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Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencing off-target mutations due to sgRNA recognizing highly homologous DNA sequence elsewhere in the genome. Whole-genome sequencing (WGS) can be used to identify on- and off-target mutation. Here, we describe a pipeline of analyzing WGS data using a series of open source software for analysis of off-targ

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Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Constructyotic cells, a very large number of derived technologies has emerged. Demonstrating and comparing the effectiveness of all these new technologies in entire plants is a long, tedious, and labor-intensive process that generally involves the production of transgenic plants and their analysis. Protoplas