Titlebook: Ribozymes and siRNA protocols; Mouldy Sioud Book 2004Latest edition Humana Press 2004

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CLEAR

Maxizyme Technology,ene-therapeutic agents. However, because of the limitation for cleavable sequences within the target mRNA, in some cases conventional ribozymes have failed to exhibit precise cleavage specificity. A maxizyme is the dimer of minimized ribozymes (minizymes), which can specifically cleave two distinct

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,Target-Site Selection for the 10–23 DNAzyme, abundant in mRNA, many of these potentially cleavable junctions are protected from DNAzyme activity by secondary structure. To optimise the process of target-site selection in long RNA substrates, a multiplex assay was developed for simultaneous comparative analysis of 50 or more different DNAzymes

Cardiac

Regulation of Ribozyme Cleavage Activity by Oligonucleotides,ing. Since both regulations are sequence-specific, ribozymes containing different sequences can be positively and independently controlled by a specific oligonucleotide. Furthermore, these allosteric hairpin loops have the ability to control the activities of other functional RNAs.

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Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo, .-regulatory elements and antibiotics that can modulate the binding of the transactivators at low and nontoxic doses. Here, we summarize protocols to generate cell lines expressing tetracycline-regulated ribozyme constructs.

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separate

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Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes,mple cleavage and ligation reactions mediated by minimal hammerhead and hairpin ribozymes under standard experimental conditions. Information is also provided to help researchers recognize and interpret more complex reaction kinetics that can be observed for ribozyme-sequence variants under a variety of reaction conditions.

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Analysis of Global Conformational Transitions in Ribozymes,s and fluorescence resonance energy transfer (FRET) can be used to analyze the global conformation in solution, and to study the ion-induced folding processes. The FRET approach can also be extended into the time domain to analyze conformational equilibria, and increasingly to the single-molecule level.