Titlebook: Ribozymes and siRNA protocols; Mouldy Sioud Book 2004Latest edition Humana Press 2004

gimmick

肿块

Analysis of Global Conformational Transitions in Ribozymes,zymes contain one or more helical junctions, that act as key architectural elements in the structures. A combination of comparative gel electrophoresis and fluorescence resonance energy transfer (FRET) can be used to analyze the global conformation in solution, and to study the ion-induced folding p

cushion

极小

inflate

Selection In Vitro of Allosteric Ribozymes,these distinct functional classes may be combined to generate multipartite ribozymes in which the catalytic and ligand-binding elements act coordinately. In this chapter, we describe a procedure known as allosteric selection, in which an RNA catalyst in conjunction with a random sequence domain is s

卧虎藏龙

ULCER

Virtues

Ribozyme Expression Systems,h level of production of ribozymes in vivo. The engineered tRNA.-driven ribozymes, based on computer-predicted secondary structure, were relatively stable and were transported to the cytoplasm, where they could be colocalized with their target RNA. The activity of the exported ribozymes was signific

FUSE

vanquish