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Titlebook: Rhodopsin; Methods and Protocol Valentin Gordeliy Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins,l circuits would enable powerful, causal investigations of neural connectivity and function at single-cell level and provide insights into how neural circuits operate. Such single-cell resolution optogenetics in neuron populations requires precise sculpting of light and subcellular targeting of opto
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Electrophysiological Characterization of Microbial Rhodopsin Transport Properties: Electrometric an based usually on measurements of electrical current through a planar bilayer lipid membrane (BLM) with proteoliposomes attached to the BLM surface at one side of the membrane. Here, we describe the measurements of the pumping activity of bR and channelrhodopsin 2 (ChR2) with special attention to th
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Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments,ar, the use of microbial rhodopsins enables remote control of excitable-cell activity by light. The electrophysiological characterization of microbial rhodopsins is inevitable for the development of variants, which further advance optogenetic applications. Therefore, we provide a detailed descriptio
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Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications,tics) and reading-out (calcium, voltage, and neurotransmitter indicators) neural activity as well as precision expression of these reagents using virally mediated delivery. With few exceptions, these powerful approaches are derived from naturally occurring molecules that are sourced from diverse org
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In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins,e different types are known: light-activated guanylyl cyclase opsins (Cyclop) in fungi, light-inhibited two-component guanylyl cyclase opsins (2c-Cyclop) in green algae, and rhodopsin phosphodiesterases (RhoPDE) in choanoflagellates. They are integral membrane proteins with eight transmembrane helic
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Book 2022 as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful .Meth
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