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Titlebook: Rho GTPases; Methods and Protocol Francisco Rivero Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer Nature 20

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发表于 2025-3-21 16:23:31 | 显示全部楼层 |阅读模式
书目名称Rho GTPases
副标题Methods and Protocol
编辑Francisco Rivero
视频video
概述Includes cutting-edge methods and protocols for the study of Rho GTPases.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Rho GTPases; Methods and Protocol Francisco Rivero Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer Nature 20
描述This detailed book expands upon the previous edition with a collection of methods for those performing experimental work on small GTPases of the Rho family. Split into four sections, the volume explores computational modeling and imaging procedures, biochemical methods related to post-translational modifications of Rho GTPases as well as some high throughput methods, functional assays that allow for monitoring the consequences of manipulating Rho GTPases in a variety of cell types and cell biology processes, and techniques specifically designed for studies in selected non-mammalian model organisms (zebrafish, social ameba, plants and algae). Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls. .Authoritative and up-to-date, .Rho GTPases: Methods and Protocols, Second Edition. constitutes an invaluable tool for all those with an interest in this remarkable family of signaling proteins..
出版日期Book 2018Latest edition
关键词Computational modeling; Rho signaling dynamics; Signaling proteins; High throughput methods; Pharmacolog
版次2
doihttps://doi.org/10.1007/978-1-4939-8612-5
isbn_softcover978-1-4939-9343-7
isbn_ebook978-1-4939-8612-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

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Francisco RiveroIncludes cutting-edge methods and protocols for the study of Rho GTPases.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/830135.jpg
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https://doi.org/10.1007/978-1-4939-8612-5Computational modeling; Rho signaling dynamics; Signaling proteins; High throughput methods; Pharmacolog
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Parallel High-Resolution Imaging of Leukocyte Chemotaxis Under Agarose with Rho-Family GTPase BiosenGTPases in this context using Förster resonance energy transfer (FRET)-based biosensors. The specific experimental steps outlined in this chapter include how to (1) set up the under-agarose assay, (2) optically pattern chemoattractant gradients, (3) image cells, and (4) conduct basic image analysis
发表于 2025-3-22 22:15:19 | 显示全部楼层
Screening for Rho GTPase Modulators in Actin-Dependent Processes Exemplified by Phagocytosis the subcellular localization of a library of Rho GTPase modulators; (2) assessing endogenous levels of expression of the suitably localized candidates in the cell type of interest; and (3) validating the functional relevance of the identified candidates by siRNA, followed by determining the effects
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