Titlebook: Rhinoviruses; Methods and Protocol David A. Jans,Reena Ghildyal Book 2015 Springer Science+Business Media New York 2015 high-end imaging te

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Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating, conditions, the dynamic processes that take place during the early steps of virus infection. We cover two important issues of rhinovirus research, the kinetics of directional RNA release, and virus-receptor interactions exemplified by using human rhinovirus type A2 (HRV-A2) as a model.

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Reverse Genetics System for Studying Human Rhinovirus Infections,ng the artificial cDNA copy of a full-length HRV genome, identifying the infectious cDNA clone isolates, and selecting the most vigorous cDNA clone isolate to serve as the standard parental clone for future molecular genetic study of the virus.

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Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples, respiratory pathogens in most, if not all, clinical microbiology laboratories. Molecular assays are significantly more sensitive than traditional culture-based and serological methods. This advantage has led to the recognition that HRV infections are common causes for not only upper airway symptoms

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Molecular Genotyping of Human Rhinovirus by Using PCR and Sanger Sequencing,at HRV is also the major viral cause of asthma exacerbations. Genotypic assignment and identification of HRV types are of significant value in the investigation of type-associated differences in disease outcomes, transmission, and epidemiology. Here, we describe a genotyping process involving two se

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Growth of Human Rhinovirus in H1-HeLa Cell Suspension Culture and Purification of Virions,ficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and maki

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Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Ill culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells

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