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Titlebook: Rhinoviruses; Methods and Protocol David A. Jans,Reena Ghildyal Book 2015 Springer Science+Business Media New York 2015 high-end imaging te

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书目名称Rhinoviruses
副标题Methods and Protocol
编辑David A. Jans,Reena Ghildyal
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Rhinoviruses; Methods and Protocol David A. Jans,Reena Ghildyal Book 2015 Springer Science+Business Media New York 2015 high-end imaging te
描述.Rhinoviruses: Methods and Protocols .highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Rhinoviruses: Methods and Protocols .seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic app
出版日期Book 2015
关键词high-end imaging techniques; human rhinovirus; human rhinovirus research; molecular genotyping; protease
版次1
doihttps://doi.org/10.1007/978-1-4939-1571-2
isbn_softcover978-1-4939-5533-6
isbn_ebook978-1-4939-1571-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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A Protocol to Express and Isolate HRV16 3C Protease for Use in Protease Assays, transformation of bacterial cells with a commercially available cDNA plasmid which can be adapted to use for 3C proteases from any other HRV serotypes. The extracted, active 3C protease can then be used for in vitro protease assays.
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Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detite-directed mutagenesis PCR to introduce the sequence for the HA antigen onto either the C or N termini of 2A protease while keeping the relevant internal cleavage sites intact. The new viral product is then cloned into a wild-type HRV16 plasmid and transfected into Ohio Hela cells to produce recombinant virus.
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Mouse Models of Rhinovirus Infection and Airways Disease,sponses, for representative viruses from two of these groups: (1) infection of unmodified Balb/c mice with minor group rhinovirus serotype 1B (RV-1B) and (2) infection of transgenic Balb/c mice with major group rhinovirus serotype 16 (RV-16).
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Book 2015search in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chroni
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