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Titlebook: Rheumatoid Arthritis; Methods and Protocol Shuang Liu Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Autoimmu

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楼主: relapse
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Generation of Specific Aptamersherapeutic molecules with high affinity and specificity, and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants, and ease of chemical
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Production of Lentiviral Particlesmodification can be achieved easily in immune cells, nondividing and terminally differentiated cells, including hematopoietic stem cells, neurons, and even tumor cells, which other viral vectors cannot do. The main considerations of therapeutic gene delivery using a lentiviral system are the risk of
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Mesenchymal Stem Cell Engineeringrogenitor cells to engineer cartilage implants that can be used to repair chondral and osteochondral lesions, or as trophic producers of bioactive factors to initiate endogenous regenerative activities in the arthritic joint. Targeted gene therapy might further enhance the capacity of MSC for chondr
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Human Xenograft Modelaneously. The same patient-derived peripheral blood mononuclear cell chimerism can be successfully achieved by intraperitoneal engraftment. This xenograft model is able to be used for initial screening of human target-specified biologics.
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Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis Systemcult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using cell-free protein synthesis method with liposome and dialysis cup. Here we introduce practical and efficient integrated procedures to produce large amount of proteoliposome antigen for anti-membrane protein antibody development.
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Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreentibody profiling assay using cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitively detect interaction between recombinant antigen protein and autoantibody and efficiently analyze autoantibody profiling in patients’ sera.
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