Titlebook: Rheumatoid Arthritis; Methods and Protocol Shuang Liu Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Autoimmu

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Book 2018ting and avoiding known pitfalls.. Authoritative and cutting-edge, .Rheumatoid Arthritis: Methods and Protocols .aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study..

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RNA Interference Ex Vivostration of siRNA or shRNA has been a significant advance in preclinical research on RA. In this chapter, the basic techniques for gene silencing in human-derived peripheral T cells using liposome-dependent siRNA transfection and lentiviral-mediated shRNA delivery, aiming at gene silencing of therapeutic targets, are introduced.

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Lentiviral-Mediated Systemic RNA Interference In Vivoow to produce concentrated shRNA-encoding lentiviral particles. Here, systemic in vivo application of lentivirus, including viral quantification prior to injection, intraperitoneal injection, and quantification of integrated provirus, is introduced.

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