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Titlebook: Reverse Phase Protein Arrays; From Technical and A Tesshi Yamada,Satoshi S. Nishizuka,Lance A. Liotta Book 2019 Springer Nature Singapore P

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发表于 2025-3-21 17:03:17 | 显示全部楼层 |阅读模式
书目名称Reverse Phase Protein Arrays
副标题From Technical and A
编辑Tesshi Yamada,Satoshi S. Nishizuka,Lance A. Liotta
视频video
概述Guidance on handling of samples for stable data generation.Explanation of the role of RPPA data in integrative analyses.Expert opinions and updates on recent results from RPPA
丛书名称Advances in Experimental Medicine and Biology
图书封面Titlebook: Reverse Phase Protein Arrays; From Technical and A Tesshi Yamada,Satoshi S. Nishizuka,Lance A. Liotta Book 2019 Springer Nature Singapore P
描述.This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility..
出版日期Book 2019
关键词Reverse-phase protein array; Technology; Drug discovery; Biomarkers; Integrative genomics; RPPA; microarra
版次1
doihttps://doi.org/10.1007/978-981-32-9755-5
isbn_softcover978-981-32-9757-9
isbn_ebook978-981-32-9755-5Series ISSN 0065-2598 Series E-ISSN 2214-8019
issn_series 0065-2598
copyrightSpringer Nature Singapore Pte Ltd. 2019
The information of publication is updating

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发表于 2025-3-21 20:35:57 | 显示全部楼层
0065-2598 updates on recent results from RPPA.This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world wh
发表于 2025-3-22 04:28:50 | 显示全部楼层
Solid Pin Protein Array Printing Platforms, nitrocellulose slide that is optimized for the particular specimen type and protein detection method. Herein we present the nuances of RPPA fabrication and study design using a solid pin arrayer and nitrocellulose-coated slides.
发表于 2025-3-22 04:49:47 | 显示全部楼层
Using Reverse Phase Protein Array (RPPA) to Identify and Target Adaptive Resistance,resistance and use of RPPA in understanding resistance mechanisms and identification of effective drug combinations. We further demonstrate that this novel information is resulting in biomarker-driven trials aimed at targeting adaptive resistance and improving patient outcomes.
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发表于 2025-3-22 16:00:27 | 显示全部楼层
High Precision RPPA: Concept, Features, and Application Performance of the Integrated Zeptosens Plactional states in a highly parallel manner even when samples exhibit a complex matrix like in tumor tissues and are available only in very limited amounts. Ideally the full workflow from sample preparation to data visualization shall be covered..This book chapter describes the key elements of the in
发表于 2025-3-22 19:29:02 | 显示全部楼层
Solid Pin Protein Array Printing Platforms,se-coated glass slides. Reverse phase protein arrays address the challenge of quantifying low-abundance proteins and posttranslationally modified proteins in cellular lysates and body fluids. RPPA technology is ideally suited to biomarker discovery, signal pathway profiling, functional phenotype ana
发表于 2025-3-22 22:49:46 | 显示全部楼层
Analytical Platforms 1: Use of Cultured Cells and Fluorescent Read-Out Coupled to NormaCurve Normal quality control an RPPA platform. Readout of the arrays uses near-infrared fluorescence labeling and data normalization is performed using the bioinformatics package NormaCurve..In the first part, we will describe the advantages, drawbacks, and different applications of cell line material for RPPA.
发表于 2025-3-23 05:10:46 | 显示全部楼层
,Combining the “Sibling Technologies” of Laser Capture Microdissection and Reverse Phase Protein Mico overcome the challenge of quantifying low-abundance proteins in heterogeneous tissues. Combining both technologies provides both unique opportunities and unique challenges. Enabling the unprecedented resolution of the activation state of labile biomarkers, such as phosphorylated cell signaling pro
发表于 2025-3-23 05:51:32 | 显示全部楼层
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