书目名称 | Reverse Phase Protein Arrays |
副标题 | From Technical and A |
编辑 | Tesshi Yamada,Satoshi S. Nishizuka,Lance A. Liotta |
视频video | |
概述 | Guidance on handling of samples for stable data generation.Explanation of the role of RPPA data in integrative analyses.Expert opinions and updates on recent results from RPPA |
丛书名称 | Advances in Experimental Medicine and Biology |
图书封面 |  |
描述 | .This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.. |
出版日期 | Book 2019 |
关键词 | Reverse-phase protein array; Technology; Drug discovery; Biomarkers; Integrative genomics; RPPA; microarra |
版次 | 1 |
doi | https://doi.org/10.1007/978-981-32-9755-5 |
isbn_softcover | 978-981-32-9757-9 |
isbn_ebook | 978-981-32-9755-5Series ISSN 0065-2598 Series E-ISSN 2214-8019 |
issn_series | 0065-2598 |
copyright | Springer Nature Singapore Pte Ltd. 2019 |