Titlebook: Retinoids; Methods and Protocol Hui Sun,Gabriel H. Travis Book 2010 Springer Science+Business Media, LLC 2010 Transport.Vitamin.Vivo.biolog

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Diet in Vitamin A Research,l. By using a well-formulated purified diet with an exact amount of vitamin A, retinoid status in rodents can be closely controlled to create specific physiological conditions that represent the wide range of vitamin A status present in human populations.

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Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigmenlture model is also described whereby a human RPE cell line that is otherwise devoid of bisretinoid lipofuscin compounds is employed and synthesized A2E is delivered to the cells. This approach allows for a population of RPE cells that have accumulated the lipofuscin fluorophore A2E in addition to A2E-free cells.

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Book 2010etinoids.In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies.Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and

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In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists,e the degree to which different ligands can act as an agonist or an inverse agonist to gain insight into the ligand-binding pocket of cone opsins and differences between the different classes of opsins. The understanding of the effects of ligands on cone opsin activity can potentially be applied to future therapeutic agents targeting opsins.

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