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Titlebook: Research Methods in Neurochemistry; Volume 1 Neville Marks,Richard Rodnight Book 1972 Plenum Press, New York 1972 nervous system.neurobiolo

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Isolation of Brain Cell Nuclei of the subcellular distribution of a substance of almost certain cytoplasmic localization, it is often sufficient to monitor (for purposes of estimating recoveries) the concentration of that substance in a crude “nuclear pellet” obtained by low-speed centrifugation of a sucrose homogenate. From mos
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Ethanolamine Plasmalogenshe total phospholipids in some subcellular fractions, the ethanolamine plasmalogens account for about one-third in the myelin, one-fifth in the microsomes, and one-eighth in the mitochondria. The following procedures are used in our laboratories for determinations of the amount and composition of th
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Mucopolysaccharides and Glycoproteinsarides and glycoproteins (Brimacombe and Webber, 1964; Gottschalk, 1972; Neufeld and Ginsburg, 1966; Quintarelli, 1968; Rossi and Stoll, 1968; Hunt, 1970; Balazs, 1970; Roy and Trudinger, 1970; Marshall and Neuberger, 1970). Many of the standard works on carbohydrate chemistry and biochemistry also
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Enzymes Involved in the Catalysis of Catecholamine Biosynthesiswas confirmed with the use of radioactive labeled precursors. Three enzymes are involved in the catalysis of norepinephrine biosynthesis: tyrosine hydroxylase (TH), dopa decarboxylase (DDC), and dopamines-hydroxylase (D.H; a fourth enzyme, namely, phenylethanolamine-.-methyltransferase (PNMT) cataly
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Ethanolamine Plasmalogensomes, and one-eighth in the mitochondria. The following procedures are used in our laboratories for determinations of the amount and composition of the ethanolamine plasmalogens. (reviewed by Horrocks, 1972).*
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