Titlebook: Reporter Gene Imaging; Methods and Protocol Purnima Dubey Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 fluo

plasma-cells

Hypoxia-Induced Reporter Genes with Different Half-Lives,ith different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.

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Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-Sensitive Nano-Aggregation Tadiation signals in targeted cells and thus enables the detection of apoptosis using PET, a sensitive and clinically practical technique. This protocol describes the chemical synthesis, radiolabeling and PET imaging of apoptosis using this probe.

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Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes,t that lack the necessary resources and infrastructure. Here, we provide a description of the materials and procedures necessary to conduct a Cerenkov luminescence imaging experiment using a variety of imaging instrumentation, radionuclides, and animal models.

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Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent I the methods for construction of a plasmid reporter gene vector expressing a fluorescent and a bioluminescent gene and its validation by in vitro assays in mammalian cells as well as by noninvasive imaging methods in small animal models.

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978-1-4939-9306-2Springer Science+Business Media, LLC, part of Springer Nature 2018

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