Titlebook: Recombineering; Methods and Protocol Christopher R. Reisch Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 CRI

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CRISPR/Cas-Mediated Genome Editing of ,o proven to be functional chassis for the discovery and production of bioactive compounds and enzymes. However, genetic engineering of . is often laborious and time-consuming. Here we describe protocols for CRISPR/Cas-mediated genome editing of .. Starting from the design and assembly of all-in-one

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Cas9 Nickase-Based Genome Editing in , efficiency is quite low and, therefore, metabolically engineered strains with increased efficiency can decrease both the overall cost and time required for biofuel production. Traditional genetic tools are inefficient, expensive, and time-consuming, but recent developments in the use of CRISPR-Cas

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Scarless Recombineering of Phage in Lysogenic State,ls without a selection marker is feasible due to its high recombination frequency, estimated as more than 40% after six cycles. The method enables scarless editing of the genome of a bacteriophage in 4–5 days.

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Recombineering-Mediated Genome Editing in Burkholderiales Strains,029, recently identified as ., were identified for efficient genetic manipulation in the native strain and several other Burkholderiales strains. In this chapter, we describe a detailed protocol for genome engineering in Burkholderiales strains via the Redγ-Redαβ7029 recombineering and Cre/loxP site-specific recombination.

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Book 2022 topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions t