Titlebook: Recombineering; Methods and Protocol Christopher R. Reisch Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 CRI

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书目名称Recombineering影响因子(影响力)




书目名称Recombineering影响因子(影响力)学科排名




书目名称Recombineering网络公开度




书目名称Recombineering网络公开度学科排名




书目名称Recombineering被引频次




书目名称Recombineering被引频次学科排名




书目名称Recombineering年度引用




书目名称Recombineering年度引用学科排名




书目名称Recombineering读者反馈




书目名称Recombineering读者反馈学科排名

Pillory

Lambda Red Recombineering of Bacteriophage in the Lysogenic State,genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3–5 days.

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Recombineering-Mediated Genome Editing in Burkholderiales Strains, also closely related Gram-negative bacteria for efficient genome editing. However, some distant bacterial species like Burkholderiales strains require host-specific Redα/Redβ recombinase pair for highly efficient genome editing. A pair of recombinases Redαβ7029 from the Burkholderiales strain DSM 7

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High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy,chnology allows to introduce mutations within bacterial genomes in a very simple and straightforward way. This technology was initially developed for . but was later extended to other organisms of interest, including the environmentally and metabolically versatile .. The technology is based on three

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Hectic

Plant Gene Modification by BAC Recombineering,rried by bacterial artificial chromosomes (BACs) in .. Whereas BAC transformation provides a simple means for integration of modified genes into the genomes of animal cells to generate knock-in and knockout lines, successful application of this strategy is hampered by low frequency of homologous rec

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Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling, (DIRex). Dup-In is used for transferring existing mutations between strains, and DIRex for generating almost any type of mutation. Both techniques use intermediate insertions with counter-selectable cassettes, flanked by directly repeated sequences that enable exact and spontaneous excision of the

Barrister

Genome Editing in , Using CRISPR/Cas9 Technology,ave recently developed a CRISPR/Cas9-based genome editing system (pCasKP-pSGKP) by coupling a CRISPR/Cas9 system with the lambda Red recombination system as well as a cytidine deaminase-mediated base editing system (pBECKP) in ., enabling rapid, scarless, and efficient genetic manipulation in divers

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