Titlebook: Recombinant Glycoproteins; Methods and Protocol Steven B. Bradfute Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusi

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Production and Purification of Antibodies in Chinese Hamster Ovary Cellsal IgG antibodies from Chinese hamster ovary cells. It includes steps for cell maintenance, transient transfection, and antibody purification via protein A affinity chromatography. The methods described are intended for the production of milligram amounts of protein but can be adapted for most small- to mid-scale applications.

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978-1-0716-3668-8The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Recombinant Glycoproteins978-1-0716-3666-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Steven B. BradfuteIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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https://doi.org/10.1007/978-1-0716-3666-4Viral-encoded glycoproteins; Mammalian proteins; Carbohydrate groups; Cell-free synthesis; Glycosylation

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Book 2024h understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the

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SARS-CoV-2 S-Protein–Ace2 Binding Analysis Using Surface Plasmon Resonance and flowing monomeric Ace2. This method will allow for the determination of the rate constants for affinity, with additional analysis that is achievable using S-protein capture levels in conjunction with the sensorgram response for relative activity benchmarking.

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