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Titlebook: Recombinant Glycoprotein Production; Methods and Protocol Virgínia Picanço-Castro,Kamilla Swiech Book 2018 Springer Science+Business Media,

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书目名称Recombinant Glycoprotein Production
副标题Methods and Protocol
编辑Virgínia Picanço-Castro,Kamilla Swiech
视频video
概述Includes cutting-edge methods protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Recombinant Glycoprotein Production; Methods and Protocol Virgínia Picanço-Castro,Kamilla Swiech Book 2018 Springer Science+Business Media,
描述.This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authoritative and cutting-edge, .Recombinant Glycoprotein Production: Methods and Protocols .aims to ensure successful results in the further study of this vital field.
出版日期Book 2018
关键词Glycoengineering; Glycosylation; viral-particles; gene therapy; bifunctional antibodies
版次1
doihttps://doi.org/10.1007/978-1-4939-7312-5
isbn_softcover978-1-4939-8449-7
isbn_ebook978-1-4939-7312-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

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Platforms for Recombinant Therapeutic Glycoprotein Production,in production, since glycosylation influences yield, pharmacokinetics, biological activity, and immunogenicity. This chapter covers the general aspects of therapeutic recombinant glycoproteins and the platforms that are being employed for their production.
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Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cellsnity FVIII select and ion exchange SP-Fastflow chromatography steps without subjecting the target molecule to mechanical and temperature stress, separating impurities from rFVIII using net charge, hydrophobicity, and affinity of the molecules.
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Human Cells as Platform to Produce Gamma-Carboxylated Proteinsthe methods to generate human cells as a platform to produce gamma-carboxylated proteins, for example the coagulation factors VII and IX, are presented. From the cell line modification up to the vitamin K adaptation of the produced cells is described in the protocols presented in this chapter.
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Production of Recombinant Factor VIII in Human Cell Linesherefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation. Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.
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Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein s is challenging, time-consuming, and cell line and medium dependent. In this chapter, we present different approaches that can be used to adapt mammalian cell lines from an anchorage-dependent serum supplemented culture to a suspension serum-free culture.
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