Titlebook: Recombinant Gene Expression Protocols; Rocky S. Tuan Book 1997 Humana Press 1997

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Design of Bacterial Hosts for ,-Based Expression Vectors its derivatives . and . promoters, have been developed (e.g., . refs. .–.). In order to maximize utilization of these vectors, various . host strains have been designed which contain the .ΔM15 allele (.) necessary for α-complementation and the .. (.,.) gene, which allows for overproduction of the .

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Application of the , Promoterproduction levels (.). The . promoter is strong, easily regulated, and well characterized. Transcription of a cloned gene from a . promoter on a plasmid increases about 50-fold upon induction and the gene product can amount to 30% of the total cell protein (.–.).

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Expression and Secretion of Proteins in ,ystems, host organisms, and processing procedures have been described. Among these, . remains an important organism for production of recombinant proteins in both the laboratory and industry. This is owing to the fact that only a few organisms can satisfy all the criteria such as high growth rate, r

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Synthetic Two-Cistron Expression Systemmmalian cells. The choice of an appropriate host organism is dictated by the particular protein to be expressed, and by requirements for posttranslational modifications, solubility and yield. The primary advantages that . offers as a host organism include the ease and speed with which recombinant st

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Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translationrequire significant quantities of product. Our laboratory has been interested in this technique because the translation of nucleic acid into protein sequence allows use of the unsurpassed resolving power of 2D protein electrophoresis to analyze complex mixtures of gene products. Thus, typically we m

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Yeast Plasmidslar mechanisms governing many cellular processes in yeasts are conserved in other organisms. For example, yeasts provide a powerful system for the study of mammalian proteins. However, to study the function of a cDNA encoding a heterologous protein in yeast, the cDNA needs to be cloned in an appropr

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