书目名称 | Recombinant Gene Expression Protocols | 编辑 | Rocky S. Tuan | 视频video | | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmamm | 出版日期 | Book 1997 | 版次 | 1 | doi | https://doi.org/10.1385/0896034801 | isbn_softcover | 978-0-89603-333-7 | isbn_ebook | 978-1-59259-548-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 1997 |
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