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Titlebook: Recombinant Gene Expression Protocols; Rocky S. Tuan Book 1997 Humana Press 1997

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书目名称Recombinant Gene Expression Protocols
编辑Rocky S. Tuan
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: Recombinant Gene Expression Protocols;  Rocky S. Tuan Book 1997 Humana Press 1997
描述The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse­ quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex­ press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap­ ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmamm
出版日期Book 1997
版次1
doihttps://doi.org/10.1385/0896034801
isbn_softcover978-0-89603-333-7
isbn_ebook978-1-59259-548-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1997
The information of publication is updating

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Lawrence M. Mylin,James E. Hopper gradient. The method involves casting a layer of polyacrylamide gel containing a mixture of carrier ampholytes (low-molecular-weight synthetic polyamino—polycarboxylie acids). When an electric field is applied to such a gel, the carrier ampholytes arrange themselves in order of increasing pi from t
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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/824092.jpg
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Strategies for Gene Fusions. The fusion proteins, expressed from such operatively linked genes, often display the combined properties of the parent proteins and are used extensively in microbiology, molecular biology, immunology, and bio-technology (.–.).
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Application of the , Promoterproduction levels (.). The . promoter is strong, easily regulated, and well characterized. Transcription of a cloned gene from a . promoter on a plasmid increases about 50-fold upon induction and the gene product can amount to 30% of the total cell protein (.–.).
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Inducible Expression Cassettes in Yeast: ADH2 which are repressed by the presence of glucose. The strength of the ADH2 promoter coupled with the fact that the promoter is tightly regulated make it highly amenable for use in the expression of heterologous genes.
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Keshav K. Singh,Jack A. Heinemann involves hydrogen bonding between a few base pairs, the dissociation temperature is very low, near 5°C. Unlike . DNA ligase, T4 DNA ligase will also covalently link two blunt-ended molecules (.C), and this reaction can be used to join ends generated by enzymes that do not give sticky ends.
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