Titlebook: Recombinant Gene Expression; Reviews and Protocol Paulina Balbás,Argelia Lorence Book 2004Latest edition Humana Press 2004

难管

Copy-Control pBAC/oriV Vectors for Genomic Cloningnal BACs but, in addition, permits, on command, amplification of the BAC plasmids and cloned DNA. This system consists of (1) plasmid pBAC/oriV containing an additional replication origin, ., and (2) a host carrying the up-mutants of the . replicator gene expressed from the .-arabinose-inducible .ar

适宜

Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriVneficial feature of these vectors is that when they are not induced, they are maintained as a single-copy plasmid, and therefore, any residual expression is much more tightly regulated than for the conventional multicopy expression vectors. The simplest version of these copy-control expression vecto

幻想

Ceramic

motivate

轨道

Recombinant Protein Production in Antarctic Gram-Negative Bacteriaoduction in cold-adapted bacteria by using natural plasmids as cloning vectors..By screening some Antarctic bacteria for the presence of extrachromosomal elements, we identified three new plasmids, pMtBL from . TAC125, and pTAUp and pTADw, from .. TA144..The latter autoreplicating elements were isol

BUDGE

Recombinant Protein Production in Yeastshemicals from naturally nonproducing cells. This technology, now approx 25 yr old, is becoming one of the most important technologies developed in the 20th century..Pharmaceutical products and industrial enzymes were the first biotech products on the world market made by means of rDNA. Despite impor

flourish

Production of Recombinant Proteinsommercially viable process. In this chapter, common problems in recombinant protein production are reviewed and strategies for their solution are discussed. Such strategies include molecular biology techniques, as well as manipulation of the culture environment. Finally, specific problems relevant to different hosts are discussed (.. and .).

Osmosis

Overexpression of Chromosomal Genes in ,o modify cells to overproduce desired molecules. We describe here the methodology to modify chromosomal genes by replacing their native regulatory regions with promoter cassettes to increase or deregulate expression of chromosomal genes.

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