Titlebook: Recombinant Enzymes - From Basic Science to Commercialization; Azura Amid Book 2015 Springer International Publishing Switzerland 2015 bro

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Introduction to Recombinant Enzyme Pre-commercialization,ant enzymes and an explanation of why they should be commercialized, the factors that encourage academics to commercialize product, the methods that can be used in the commercialization process and the obstacles in the commercialization process.

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Case Study: Recombinant Bromelain Selection,at we examined were the following: (1) easy access to a DNA source; (2) broad application; (3) an enzyme size amenable to the cloning strategy; (4) available data in free online databases; (5) broad industrial application.

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Recombinant Enzyme: Cloning and Expression,This chapter will discuss the general strategies of cloning recombinant enzymes from identifying a suitable DNA template to choosing the correct host expression system. However, there is no standard operating procedure for cloning a recombinant enzyme. The best strategies should be based on the researcher’s creativity and experiences.

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Recombinant-Enzyme Fermentation,This chapter presents an overview of the issues involved in the fermentation of . harboring a transgene. The successful production of a heterologous enzyme requires the thoughtful integration and optimization of media formulations, physical factors associated with fermentation, host specificities and induction strategies.

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