Titlebook: Recombinant DNA Research and Viruses; Cloning and Expressi Yechiel Becker,Julia Hadar (Managing Editor) Book 1985 Martinus Nijhoff Publishi

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Book 1985es and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus ge

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Cloning of Human Oncogenesng plasmid with sequences of human c-. mRNA has been isolated which can transform 3T3 fibroblasts. By somatic cell hybrid analysis and . hybridization, the chromosomal location of each of these oncogene homologues was determined, and compared to the chromosomal breakpoints in translocations in specific malignancies.

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Cloned DNA as a Substrate of Bacterial Recombination Systemcture and function of the cloned fragment at its place of origin. Measures must therefore be taken to minimize deletions and rearrangements of cloned DNA in the host bacteria. The nature of the elements which affect the integrity of cloned DNA is the scope of this manuscript.

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