Titlebook: Receptor Signal Transduction Protocols; R. A. J. Challiss Book 1997 Springer Science+Business Media New York 1997

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Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Bindinghe most part been limited to study of the structure-activity relationships (SAR) of agonists and antagonists at the individual receptors. For a particular receptor, these SAR data were obtained via synthesis of series of chemically modified hgands, followed by the determination of their binding acti

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Approaches to the Stable Transfection of G Protein-Coupled Receptorsnction. The use of stably transfected cell lines enables a receptor to be studied in the absence of other related receptor subtypes. With this technique, molecular determinants of ligand binding can be identified; signal transduction pathways of a particular receptor can be delineated; interactions

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Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channelsde variety of proteins (.–.). Transient transfections are especially practical for the expression of recombinant multisubunit complexes because their expression needs the prior knowledge and selection of which of a set of subunits are necessary to form functional heteromultrmers with distinct featur

deficiency

The Generation of Receptor-Selective Antibodieshan pharmacological probes. They are often the only probes available that can discern proteins with similar structural and pharmacological properties, such as distinct isoforms or splice-variants of a receptor family. Furthermore, antibodies may be utilized to determine a wide range of a receptor’ c

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lmmunocytochemical Methods for Investigating Receptor Localizationhe availability of immunochemical probes to simultaneously visualize cellular ultrastructure, such as intracellular organelles, cytoskeleton, proteins involved in signal transduction, and markers of cellular differentiation. The application of immunocytochemical methods, in conjunction with experime

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exceed

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Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Couplingoteins with altered structures that can be studied for changes in biochemical properties. The most common way to do structure/function studies is by site-directed mutagenesis, in which a particular residue in a protein is selected and systematically altered. Because of the labor-intensive nature of

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