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Titlebook: Receptor Binding Techniques; Anthony P. Davenport Book 2005Latest edition Humana Press 2005 PCR.cell.enzymes.hybridization.ion channels.li

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Immunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application tated using examples of how site-directed polyclonal antisera can be used to distinguish between subtypes of G protein-coupled receptors (GPCRs; endothelin ET. and ET.), (.) in sections of human tissue or whole-body sections of mice, as well as mapping previously designated orphan receptors (oGPCR) r
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Live Cell Imaging of G Protein-Coupled Receptors, analysis of the transport of GPCRs was the molecular cloning of the green fluorescent protein (GFP) from the jellyfish . by Prasher and coworkers (., .). Site-directed mutagenesis yielded derivatives with higher photostability, improved quantum yield, and different excitation/emission spectra (.– .
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Imaging and Characterization of Radioligands for Positron Emission Tomography Using Quantitative Phon sensitive film (.) (.. and .). The major advantage of phosphor screens over film is the greatly increased sensitivity with exposure times reduced by at least one order of magnitude (.,.). This is essential for short-lived radionuclides—such as .F and .1C, with half-lives of 109.8 and 20.4 min, re
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Book 2005Latest editionfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
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Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels,ome available before molecular and immunological reagents have been developed. A pharmacologically active agent may be synthesized before the endogenous ligand for its binding site has been identified, and autoradiographic methods may help elucidate the site of action of such agents.
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