书目名称 | Receptor Binding Techniques | 编辑 | Anthony P. Davenport | 视频video | | 概述 | Includes supplementary material: | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. | 出版日期 | Book 2005Latest edition | 关键词 | PCR; cell; enzymes; hybridization; ion channels; lipide; microscopy; protein; radiography; research | 版次 | 2 | doi | https://doi.org/10.1385/1592599273 | isbn_softcover | 978-1-61737-556-9 | isbn_ebook | 978-1-59259-927-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Humana Press 2005 |
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