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Titlebook: Receptor Binding Techniques; Anthony P. Davenport Book 2005Latest edition Humana Press 2005 PCR.cell.enzymes.hybridization.ion channels.li

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发表于 2025-3-21 18:21:30 | 显示全部楼层 |阅读模式
书目名称Receptor Binding Techniques
编辑Anthony P. Davenport
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Receptor Binding Techniques;  Anthony P. Davenport Book 2005Latest edition Humana Press 2005 PCR.cell.enzymes.hybridization.ion channels.li
描述A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
出版日期Book 2005Latest edition
关键词PCR; cell; enzymes; hybridization; ion channels; lipide; microscopy; protein; radiography; research
版次2
doihttps://doi.org/10.1385/1592599273
isbn_softcover978-1-61737-556-9
isbn_ebook978-1-59259-927-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2005
The information of publication is updating

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发表于 2025-3-21 21:34:10 | 显示全部楼层
Anthony P. Davenport,Rhoda E. Kucated the common press to coin the phrase ‘Dr. med. computer‘. Papers printed under this heading rebuffed the majority of physiCians for many years. Today we kno978-3-540-50317-0978-3-642-48706-4Series ISSN 0172-7788
发表于 2025-3-22 04:22:33 | 显示全部楼层
Peter Johnström,Tim D. Fryer,Hugh K. Richards,Olivier Barret,Anthony P. Davenportescribe the prototypes. This material is usually in the form of sample rules and a description of the process for applying the rules. The third location of the 978-94-010-5746-2978-94-011-3923-6Series ISSN 0923-6716 Series E-ISSN 2199-1057
发表于 2025-3-22 07:22:30 | 显示全部楼层
escribe the prototypes. This material is usually in the form of sample rules and a description of the process for applying the rules. The third location of the 978-94-010-5746-2978-94-011-3923-6Series ISSN 0923-6716 Series E-ISSN 2199-1057
发表于 2025-3-22 12:20:48 | 显示全部楼层
Receptor Databases and Computational Websites for Ligand Binding,rotein and nucleotide sequences, classification, experimental results, and computational tools for modeling interactions. A number of websites in the public domain provide useful data-mining tools and contain information on specific families of receptors or receptor subfamilies, such as the G protei
发表于 2025-3-22 13:14:03 | 显示全部楼层
,Identification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR® Assays,25 billion in worldwide pharmaceutical sales. Therefore, considerable effort has been made to identify cognate ligands for oGPCRs and functionally characterize these receptors in order to elucidate their physiological and therapeutic relevance.
发表于 2025-3-22 18:11:59 | 显示全部楼层
mRNA, in pathological states and the possible modes of action of drugs used to treat such conditions (.–.). Changes at the molecular level to alter mRNA expression represent rapid changes within a cell; therefore, it can be envisaged that such studies on human biopsy and post mortem tissue will lead to a
发表于 2025-3-22 21:53:57 | 显示全部楼层
Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established aes, and has been very successful. More than 45 receptors have been ″de-orphanized″ or paired with their cognate ligand, with nearly half of these putative endo- genous transmitters turning out to be peptides (..). The number of pairings continues to increase. It is estimated that about 70 of the rem
发表于 2025-3-23 04:45:42 | 显示全部楼层
Live Cell Imaging of G Protein-Coupled Receptors,ffinity, signal transduction, or intracellular trafficking) of GPCRs are not, or are only slightly, altered (.–.). Thus, GFP and its derivatives have been widely applied as fluorescent probes to visualize trafficking of GPCRs in real time, to analyze GPCRs‘ mobility by fluorescence recovery after ph
发表于 2025-3-23 08:00:20 | 显示全部楼层
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