Titlebook: Radiation Cytogenetics; Methods and Protocol Takamitsu A. Kato,Paul F. Wilson Book 2019 Springer Science+Business Media, LLC, part of Sprin

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Book 2019d-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a se

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G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity,ically, asynchronous growing cells are irradiated with less than 1 Gy and allowed 0.5–1 h for cells in mitosis, at the time of irradiation, to transit into G1. Later, the G2 phase cells, at the time irradiation, are blocked by colcemid for 1–4 h at metaphase. Cells are collected by standard hypotoni

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Sister Chromatid Exchange as a Genotoxic Stress Marker,NA’s semiconservative replicative nature. Thymidine analogues such as 5′-bromodeoxyuridine (BrdU) and ethynyldeoxyuridine (EdU) are incorporated into the newly synthesized DNA for two cell cycles. The addition of Colcemid to the culture blocks and synchronizes cells at mitosis, and conventional cyto