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Titlebook: Rab GTPases; Methods and Protocol Guangpu Li,Nava Segev Book 2021Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview,tly to analyze regulation, localization, interactions, and function of Rab GTPases and their roles in human disease. We discuss contributions of these methods to new insights into Rabs, as well as their future use in addressing open questions in the field of Rab biology.
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Qualitative and Quantitative Assessment of the Role of Endocytic Regulatory and/or Rab Proteins on of these proteins on mitochondria by quantifying their effect on mitochondrial morphology. It is our goal to provide simple protocols that may prove useful for researchers new to the realm of endocytic regulatory proteins and mitochondrial homeostasis.
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Methods for Establishing Rab Knockout MDCK Cells,ls) by using Cas9-mediated genome editing technology to analyze the function of each Rab or closely related Rabs in cell viability (or growth), organelle morphology, and epithelial morphogenesis. In this chapter, we describe the procedures for generating Rab-KO MDCK II cells in detail.
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Book 2021Latest editionover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-b
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