Titlebook: Rab GTPases; Methods and Protocol Guangpu Li,Nava Segev Book 2021Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview, undergo distinct changes in conformation between their GTP- and GDP-bound forms and cycle between the cytoplasm and membranes with the aid of their upstream regulators. When activated on the membrane in the GTP-bound form, they recruit their downstream effectors, which include components of vesicul

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Imaging of Spatial Cycling of Rab GTPase in the Cell,to localize on specific membranes in cells. It remains elusive how the distinct spatial distribution of Rab GTPases in the cell is regulated. To make a global assessment on the subcellular localization of Rab1, we determined kinetic parameters of the spatial cycling of Rab1 in live cells using photo

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,Determination of the Rab27–Effector Binding Affinity Using a High-Throughput FRET-Based Assay, their GTP-bound state. In recent years, a number of studies has suggested roles for Rab27–effector protein interactions in the development of cancer cell invasion and metastasis, and immune and inflammatory responses. Here we develop an in vitro fluorescence resonance energy transfer (FRET)-based p