Titlebook: RNAi and Plant Gene Function Analysis; Methods and Protocol Hiroaki Kodama,Atsushi Komamine Book 2011 Springer Science+Business Media, LLC

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Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs,RNAs with a characteristic hairpin structure and regulate gene activity by targeting mRNA transcripts for cleavage or translational repression. Efficient and reliable detection and quantification of miRNA expression has become an essential step in understanding their specific roles. The expression l

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Large-Scale Sequencing of Plant Small RNAs, interfering RNA (siRNA) and miRNA are two classes of DCL-dependent small RNAs known to affect phenotype, developmental regulation, and various traits in plants. These small RNAs function by selectively repressing gene expression mainly by guiding cleavage, resulting in degradation of target transcr

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Comparative Analysis of Phosphoprotein Expression Using 2D-DIGE,eful to compare protein expression between wild-type and RNAi plants. 2D-DIGE (difference gel electrophoresis) was developed to perform quantitative proteomics of two or more samples. In this method, proteins are fluorescently labeled by Cy2, Cy3, or Cy5 and are mixed and subjected to electrophoresi

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Heat-Inducible RNAi for Gene Functional Analysis in Plants,function by heat-inducible RNAi. This system is easy to use and complementary to those based on chemical gene inducer treatments and might be useful for both research and biotechnological applications.

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