Titlebook: RNAi and Plant Gene Function Analysis; Methods and Protocol Hiroaki Kodama,Atsushi Komamine Book 2011 Springer Science+Business Media, LLC

排他

Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of ,,fecting them with in vitro-synthesized dsRNAs. In this chapter we detail the procedure for transient gene silencing in protoplasts using synthetic dsRNAs and provide examples of approaches for subsequent functional analyses.

手榴弹

dysphagia

Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants,d lines of transgenic plants (CG>CNG>CHH), while the methylation levels were low in nonsilenced, over-expressing lines. Through grafting, RNA silencing was induced in the non-silenced scions from silenced rootstocks; however, the methylation level of DNA in the scions did not increase.

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搜寻

Aesthete

Mitigate

evince

流逝

Using Nuclear Run-On Transcription Assays in RNAi Studies,led transcripts that then can be hybridized to filter-bound, cold, excess single-stranded DNA probes representing genes of interest. The protocol presented here streamlines, adapts, and optimizes nuclear run-on transcription assays for use in RNAi studies.

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