Titlebook: RNA‘Protein Interaction Protocols; Susan R. Haynes Book 19991st edition Humana Press 1999

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An ,-Based Genetic Strategy for Characterizing RNA Binding Proteins, have simplified the analysis of RNA-protein interactions. Nevertheless, a thorough analysis of an RNA-BP is not a trivial task, and, at present, there are only a few RNA-BPs that have been characterized in detail.

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In Vitro Genetic Analysis of RNA-Binding Proteins Using Phage Display,NA. To gain more insight into RNA-protein interaction, several genetic systems have been developed in the last few years (.–.) (.. and .). The system described in this chapter is based on in vitro genetics. This means that the selective step (in this case, the binding of an RNA-BP to RNA) occurs outside of a living organism, in the test tube.

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Book 19991st editionriety of RNA–protein interactions to regulate the expression of their genes. This is particularly true for eukaryotes, since newly synthesized messenger RNA must be extensively modified and transported to the cytoplasm before it can be used for protein synthesis. The realization that posttranscripti

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Labeling and Purification of RNA Synthesized by In Vitro Transcription,vitro (.–.). Early methods of in vitro transcription included the use of eukary-otic cell extracts or . RNA polymerase to transcribe DNA templates containing the appropriate promoter. Ideally, however, the optimal in vitro transcription system should require simple buffer components without need for

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The Methylene Blue Mediated Photocrosslinking Method for Dectection of Proteins that Interact with lves illuminating a mixture of protein and nucleic acid with light of a suitable wavelength to induce photochemical reactions that result in covalent linkages between the nucleic acid and the protein. The protein can be a purified or a recombinant RNA binding protein, but perhaps a more common appli