Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2023Latest edition The Editor(s) (if applicable) and The Aut

handle

concubine

古老

窗帘等

,SHAPE to Probe RNA Structure and RNA–Protein Interactions In Vitro,ranscription reaction. The number of RNA molecules with an adduct at a specific nucleotide position indicates the SHAPE reactivity of that nucleotide. Here, we describe the method for probing the structure of an RNA in a protein-free or a protein-bound state by in vitro SHAPE.

艺术

Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interactinchain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA complexes followed by subsequent extraction and quantification of the co-purified RNA.

inspired

1064-3745 ation advice from the experts.This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-pro

干涉

CLASP

A Simple Method for the Detection of Wybutosine-Modified tRNAPheGAA as a Readout of Retrograde tRNAmodified tRNA. serves as a readout of retrograde nuclear import and nuclear re-export. This simple assay can be used to determine the role of any gene product in these previously elusive tRNA trafficking events.

meditation

PALSY

Global Assessment of Protein Translation in Mammalian Cells Using Polysome Fractionation, gradient and generates the fractions. These fractions can be subjected to further RNA and protein analyses, for example, polysome profiling and mass spectrometry. Here, we present a detailed protocol of polysome fractionation for mammalian cells.