Titlebook: RNA Tagging; Methods and Protocol Manfred Heinlein Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 RNA-binding

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Plant Individual Nucleotide Resolution Cross-Linking and Immunoprecipitation to Characterize RNA-Pro (cross-linking immunoprecipitation-high-throughput sequencing). Here, we present a version of the individual nucleotide resolution cross-linking and immunoprecipitation procedure that is suitable for use in the model plant ..

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In Vivo Imaging of Mobile mRNAs in Plants Using MS2ding protein MS2 provides a useful tool to investigate intracellular trafficking of mobile mRNAs in plants. Here we describe the detailed protocol to visualize intracellular trafficking of plant mobile mRNAs by using the MS2 live-cell imaging system.

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1064-3745 expertsThis book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, an

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Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cellsreticulum (ER). Other such mRNAs are tethered to the ER by mRNA receptors. However, there has been much debate as to whether all mRNAs, regardless of their encoded polypeptide, are anchored to the ER at some low level. Here we describe a protocol to visualize ER-associated mRNAs in tissue culture ce