书目名称 | RNA Tagging | 副标题 | Methods and Protocol | 编辑 | Manfred Heinlein | 视频video | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .RNA Tagging: Methods and Protocols. serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.. | 出版日期 | Book 2020 | 关键词 | RNA-binding proteins; RNA transport; RNA-mediated editing; CRISPR; Guide RNA design; Functional RNA compl | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-0712-1 | isbn_softcover | 978-1-0716-0714-5 | isbn_ebook | 978-1-0716-0712-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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