Titlebook: RNA Spectroscopy; Methods and Protocol Véronique Arluison,Frank Wien Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein nment. The SNAP.-tag has been instrumental to study native RNA-protein complexes at single-molecule resolution in their cellular environment as efficient labeling of the RNAs and proteins of interest is essential for this colocalization single-molecule spectroscopy (CoSMoS) technique. However, remov

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Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species,gle-molecule resolution. Conventionally, the interaction between a single RNA species and multiple proteins is monitored in real time. However, comparing interactions of the same proteins with different RNA species in the same cell extract promises unique insights into RNA biology. Here, we describe

HALL

Dynamic Light Scattering Analysis on RNA Associated to Proteins, real time. Here, we describe how to analyze by DLS an RNA-protein interaction. In our frame, we studied complexes formed between RNA fragments derived from the genome of HIV-1 in association with the viral precursor Pr55.. These interactions are crucial for the specific selection of the viral genom

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Bioinformatic Application of Fluorescence-Based In Vivo RNA Regional Accessibility Data to Identify identify novel RNA: RNA interactions. Here, we document the step-by-step analysis of fluorescence data collected using an in vivo regional RNA structural sensing system (iRS.) for the purpose of identifying potential functional sites that are likely to be involved in regulatory interactions. We als

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Mapping of Posttranscriptional tRNA Modifications by Two-Dimensional Gel Electrophoresis Mass Spectn products which are further separated by nano-liquid chromatography and analyzed by MS and MS/MS. Recent developments in MS instrumentation allow the possibility to deeply explore posttranscriptional modifications. Notably, development of nano-liquid chromatography and nano-electrospray drastically

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