Titlebook: RNA Silencing; Methods and Protocol Gordon G. Carmichael Book 2005 Humana Press 2005 DNA.Vivo.gene expression.genes.molecular biology.regul

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Silencing Gene Expression with Dicer-Generated siRNA Pools,. Binding of the antisense strand to the target mRNA triggers cleavage of the mRNA (.). siRNA-mediated gene silencing is very specific, most likely because of the high specificity of nucleotide base-pairing.

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High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes,nd . as posttranscriptional negative regulators for several genes, including ., ., and . genes (.). In general miRNAs bind to the 3′ untranslated regions (UTRs) of their target mRNAs with imperfect homology (.).

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Separation of , RNA Silencing Complexes by Native Gel Electrophoresis,xes and their precursors can often be resolved and analyzed by native gel electrophoresis. Although other techniques exist for large-scale separations, native gels require very little sample, making them superior for analytical applications. We have developed a native gel system for rapidly and reli

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RNA Interference in ,,. This RNA-mediated interference, or RNAi, effectively shuts down expression of the target gene and can phenocopy loss-of-function mutations. RNAi is also remarkably potent, requiring only substoichiometric amounts of dsRNA to elicit a response (.). Another notable aspect of RNAi in . is that it is

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