| 书目名称 | RNA Silencing | | 副标题 | Methods and Protocol | | 编辑 | Gordon G. Carmichael | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. | | 出版日期 | Book 2005 | | 关键词 | DNA; Vivo; gene expression; genes; molecular biology; regulation; transcription | | 版次 | 1 | | doi | https://doi.org/10.1385/1592599354 | | isbn_softcover | 978-1-61737-568-2 | | isbn_ebook | 978-1-59259-935-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2005 |
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发表于 2025-3-21 17:06:52
