Titlebook: RNA Nanotechnology and Therapeutics; Methods and Protocol Peixuan Guo,Farzin Haque Book 2015 Springer Science+Business Media New York 2015

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Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly,important functions in the regulation of biological processes such as RNA decay, translation, or protein translocation. In bacteria, most of those noncoding RNAs have been shown to be critical for posttranscriptional control through their binding to the untranslated regions of target mRNAs. Recent e

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Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM, not removed from Okazaki fragments during DNA replication or incorporated and scattered throughout the genome, pose a perturbation to the structure and a threat to the stability of DNA. The instability of DNA is mainly due to the extra 2′-hydroxyl (OH) group of rNMPs which give rise to local struct

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Using RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection ofs, therapeutics and nanotechnology. Noncoding RNAs, such as siRNA, miRNA, ribozymes, and riboswitches, play important roles in the regulation of cellular processes. They carry out highly specific functions on a compact and efficient footprint. The properties of specificity and small size make them e

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Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases,s to the design and synthesis of nanoparticles composed mainly of RNA . bottom-up self-assembly. RNA nanoparticle is a suitable candidate for targeted delivery of therapeutics to cancer cells due to its multivalency, which allows the combination of therapeutic, targeting, and detection moieties all

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Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor,ing proper detection methods has lagged behind resulting in the lack of precise evaluation and monitoring of the systemically administered nanoparticles. RNA nanoparticles derived from the bacteriophage phi29 DNA packaging motor pRNA have emerged as a new generation of drugs for cancer therapy. Mult

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,Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screensch as nanoparticles. Functionalized particles can then be used to various aims. We took advantage of the AlphaScreen. technology for monitoring aptamer-mediated interactions. A particle bearing an aptamer contains a photosensitizer whereas another type of particle contains a chemiluminescer. Irradia