Titlebook: RNA Nanostructures; Design, Characteriza Kirill A. Afonin Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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Light-Assisted Drying for the Thermal Stabilization of Nucleic Acid Nanoparticles and Other Biologic nanoparticles (NANPs) are an example of new biological products that require refrigerated storage. Light-assisted drying (LAD) is a new processing technique to prepare biologics for anhydrous storage in a trehalose amorphous solid matrix at ambient temperatures. Small volume samples (10 μL) contain

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Obscure

Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Meltingdified RNA strands, and their mixtures. The structural properties of NA nanoparticles (NANP) generally dictate and significantly impact biological function; and thus, it is critical to extract information regarding relative stabilities of the different structural forms. The adequate stability assess

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Obsessed

Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopyusing atomic force microscopy (AFM) imaging. AFM is an indispensable tool for characterization of nucleic acid-based nanostructures with the exceptional capability of observing complexes in the range of a few nanometers. This method can visualize structural characteristics and evaluate differences b

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Synthesis of Mesoporous Silica Nanoparticles for the Delivery of Nucleic Acid Nanostructuresc material with different physiochemical properties. Mesoporous silica nanoparticles (MSNPs) are a versatile platform for the efficient delivery of nuclei acid-based materials. In this chapter, we describe the synthesis of MSNPs to efficiently transport nucleic acid nanoparticles.

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Discriminating Immunorecognition Pathways Activated by RNA Nanostructuresimmunomodulatory properties. By taking advantage of the NANPs’ unique and relatively straightforward self-assembling behavior, nucleic acid sequences can be designed from the bottom-up and specifically tailored to induce certain immune responses in mammalian cells (Johnson et al., Nucleic Acids Res

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